GeneTex
United States (US)

Ataxin 3 antibody

Overexpression
GTX115032_40247_WB_M_brain_18121410_331.jpg
GTX115032_40247_WB_B_18121410_310.jpg
GTX115032_40247_IHC_18121410_170.jpg
GTX115032_40247_20160706_2_IFA_18121410_756.jpg
GTX115032_43005_20171020_WB_18121410_494.jpg
GTX115032_43005_20171101_IFA_18121410_737.jpg

Cat No. GTX115032

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

SK-N-SH , SK-N-AS , mouse brain , human Ataxin3-transfected 293T

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ataxin 3,At3,Atx3,Jos,Mjd,Mjd1,Sca3,Atxn3

Cellular Localization

Nucleus matrix

Background

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115032_40247_WB_M_brain_18121410_331.jpg

GTX115032 WB Image

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Ataxin 3 antibody (GTX115032) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115032_40247_WB_B_18121410_310.jpg

GTX115032 WB Image

Overexpression

Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+).
10% SDS-PAGE
Ataxin 3 antibody (GTX115032) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115032_40247_IHC_18121410_170.jpg

GTX115032 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3(GTX115032) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115032_40247_20160706_2_IFA_18121410_756.jpg

GTX115032 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 protein stained by Ataxin 3 antibody (GTX115032) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX115032_43005_20171020_WB_18121410_494.jpg

GTX115032 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX115032) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115032_43005_20171101_IFA_18121410_737.jpg

GTX115032 ICC/IF Image

Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 stained by Ataxin 3 antibody (GTX115032) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169