ICC/IF analysis of U-87 MG cells treated with 100 ng of Nocodazole for 16 hours using GTX11434 Ataxin 7 antibody. Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution: 2 μg/ml