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Atp6v1al antibody

Anti-Atp6v1al antibody used in Western Blot (WB). GTX129736
Anti-Atp6v1al antibody used in IHC (Whole mount) (IHC-Wm). GTX129736
Anti-Atp6v1al antibody used in IHC (Whole mount) (IHC-Wm). GTX129736

Cat. No. GTX129736

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Atp6v1al products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Atp6v1al

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886082

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit Aa , ATP6V1A , atp6v1al , zgc:63516

Database

Research Area

DATA IMAGES

Anti-Atp6v1al antibody used in Western Blot (WB). GTX129736

GTX129736 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Atp6v1al antibody (GTX129736) diluted at 1:1000.

Anti-Atp6v1al antibody used in IHC (Whole mount) (IHC-Wm). GTX129736

GTX129736 IHC-Wm Image

Atp6v1al antibody detects atp6v1al protein at epidermal Ionocytes on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
atp6v1al antibody (GTX129736) dilution: 1:100.

Anti-Atp6v1al antibody used in IHC (Whole mount) (IHC-Wm). GTX129736

GTX129736 IHC-Wm Image

Atp6v1al antibody detects Atp6v1al protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Atp6v1al antibody (GTX129736) dilution: 1:100.

REFERENCE

Application Reference

Chen X et al. Biomedicines 2021; 9 (11) Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

Atp6v1al antibody Cat. No. GTX129736

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 4 based on 1 users reviews)
Anti-Atp6v1al antibody used in IHC (Whole mount) (IHC-Wm). GTX129736
Date : Anonymous submitted on 08-Jul-2013
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Sample : whole-mount zebrafish embryos
Blocking : commercial blocking solution, 25°C, 2Hr
Fixation : 4% paraformaldehyde
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : It detects atp6v1al protein at epidermal Ionocytes on whole-mount zebrafish embryos by immunohistochemical analysis.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169