Aurora A antibody [1F8]

Cat. No. GTX60391

Host	Mouse
Clonality	Monoclonal
Clone Name	1F8
Isotype	IgG1
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human, Rat, Monkey
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60391 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human AURKA expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6790 AURKA
UniProt: O14965 AURKA

Research Area

DATA IMAGES

GTX60391 ELISA Image

ELISA analysis of antigen using GTX60391 Aurora A antibody [1F8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60391 WB Image

WB analysis of HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate using GTX60391 Aurora A antibody [1F8].

GTX60391 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60391 Aurora A antibody [1F8].
Green : Aurora A
Blue: DRAQ5 fluorescent DNA dye

GTX60391 FACS Image

FACS analysis of K562 cells using GTX60391 Aurora A antibody [1F8].
Green : Aurora A
Purple : negative control

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty

Aurora A antibody [1F8]

Cat. No. GTX60391

Host

Clonality

Clone Name

Isotype

Application

Reactivity