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Aurora A antibody [4G10]

Anti-Aurora A antibody [4G10] used in IHC (Paraffin sections) (IHC-P). GTX60392
Anti-Aurora A antibody [4G10] used in Western Blot (WB). GTX60392
Anti-Aurora A antibody [4G10] used in ELISA (ELISA). GTX60392

Cat. No. GTX60392

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G10

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Rat, Monkey
Package
100 μl ($399)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human AURKA expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aurora A antibody [4G10] used in IHC (Paraffin sections) (IHC-P). GTX60392

GTX60392 IHC-P Image

IHC-P analysis of ovarian cancer (left) and lung cancer (right) using GTX60392 Aurora A antibody [4G10].

Anti-Aurora A antibody [4G10] used in Western Blot (WB). GTX60392

GTX60392 WB Image

WB analysis of HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate using GTX60392 Aurora A antibody [4G10].

Anti-Aurora A antibody [4G10] used in ELISA (ELISA). GTX60392

GTX60392 ELISA Image

ELISA analysis of antigen using GTX60392 Aurora A antibody [4G10].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399