GeneTex
United States (US)

Aurora A antibody, C-term

GTX29689_1101_WB_18121410_757.jpg

Cat No. GTX29689

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IP Assay dependent

Note :

IP
This antibody was deemed fit for IP under native conditions.

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX29689-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aurora Kinase A,Aik,Ark1,Aura,Btak,Ppp1R47,Stk15,Stk6,Stk7,Aurka

Cellular Localization

Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX29689_1101_WB_18121410_757.jpg

GTX29689 WB Image

WB analysis of Jurkat lysate using GTX29689 Aurora A antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer