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Aurora A antibody [C3], C-term

Overexpression
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in Western Blot (WB). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620
Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

Cat. No. GTX104620

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Aurora A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949708

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Cellular Localization

Centrosome , Spindle

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Aurora A antibody [C3], C-term used in Western Blot (WB). GTX104620

GTX104620 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody [C3], C-term (GTX104620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Aurora A antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104620

GTX104620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TW2.6 xenograft, using Aurora A(GTX104620) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-Aurora A antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104620

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE

Application Reference

Zhang Y et al. Pancreatology 2022; 22 (5) : 619-625 Aurora kinase a inhibitor MLN8237 suppresses pancreatic cancer growth.
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169