GeneTex
United States (US)

Aurora A antibody [C3], C-term

GTX104620_43033_20180110_ICC_IF_19011506_315.jpg
GTX104620_43075_20180110_ICC_IF_19011506_687.jpg
GTX104620_39820_IFA_19011506_978.jpg
GTX104620_39820_IFA_2_19011506_281.jpg
GTX104620_43033_20180202_WB_B_19011506_288.jpg
GTX104620_39820_IHC_19011506_852.jpg

Cat No. GTX104620

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Aurora A-transfected 293T

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Aurora A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aurora Kinase A,Aik,Ark1,Aura,Btak,Ppp1R47,Stk15,Stk6,Stk7,Aurka

Cellular Localization

Centrosome , Spindle

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104620_43033_20180110_ICC_IF_19011506_315.jpg

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX104620_43075_20180110_ICC_IF_19011506_687.jpg

GTX104620 ICC/IF Image

Aurora A antibody [C3], C-term detects Aurora A protein at spindle by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Aurora A stained by Aurora A antibody [C3], C-term (GTX104620) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX104620_39820_IFA_19011506_978.jpg

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX104620_39820_IFA_2_19011506_281.jpg

GTX104620 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Aurora A(GTX104620) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX104620_43033_20180202_WB_B_19011506_288.jpg

GTX104620 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody [C3], C-term (GTX104620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104620_39820_IHC_19011506_852.jpg

GTX104620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TW2.6 xenograft, using Aurora A(GTX104620) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf