GeneTex
United States (US)

Aurora A antibody

Orthogonal Overexpression
GTX134461_43215_20180615_WB_B_18121410_331.jpg
GTX134461_43215_20180622_WB_treatment_Nocodazole_18121410_194.jpg

Cat No. GTX134461

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

AURKA-transfected 293T , HeLa (100 ng/ml Nocodazole treatment for 24 hr)

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Aurora A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AIK antibody , ARK1 antibody , AURA antibody , AURKA antibody , AURORA2 antibody , BTAK antibody , PPP1R47 antibody , STK15 antibody , STK6 antibody , STK7 antibody , aurora kinase A antibody

Cellular Localization

Centrosome , Spindle

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Research Area

DATA IMAGES
GTX134461_43215_20180615_WB_B_18121410_331.jpg

GTX134461 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody (GTX134461) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX134461_43215_20180622_WB_treatment_Nocodazole_18121410_194.jpg

GTX134461 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora A antibody (GTX134461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf