GeneTex
United States (US)

Aurora A (phospho Thr288) antibody

GTX38590_20190129_IHC-P_19012901_853.jpg

Cat No. GTX38590

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:50-100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

AuroraA (phospho-Thr288) Antibody detects endogenous levels of Aurora A only when phosphorylated at threonine 288.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human AuroraA around the phosphorylation site of threonine 288 (R -T-T(p)-L-M).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aurora Kinase A,Aik,Ark1,Aura,Btak,Ppp1R47,Stk15,Stk6,Stk7,Aurka

Cellular Localization

Centriole,Centrosome,Cilium basal body,Cytoplasm,Cytoskeleton,Microtubule organizing center,Spindle pole

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX38590_20190129_IHC-P_19012901_853.jpg

GTX38590 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX38590 Aurora A (phospho Thr288) antibody.