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Aurora A (phospho Thr288) antibody

Anti-Aurora A (phospho Thr288) antibody used in Western Blot (WB). GTX50345
Anti-Aurora A (phospho Thr288) antibody used in IHC (Paraffin sections) (IHC-P). GTX50345

Cat. No. GTX50345

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from human Aurora A.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aurora A (phospho Thr288) antibody used in Western Blot (WB). GTX50345

GTX50345 WB Image

WB analysis of extracts from HT-29 cells treated with Hydroxyurea or calf intestinal phosphatase (CIP) using GTX50345 Aurora A (phospho Thr288) antibody.

Anti-Aurora A (phospho Thr288) antibody used in IHC (Paraffin sections) (IHC-P). GTX50345

GTX50345 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50345 Aurora A (phospho Thr288) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399