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Aurora A (phospho Thr288) antibody

Anti-Aurora A (phospho Thr288) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55002
Anti-Aurora A (phospho Thr288) antibody used in Western Blot (WB). GTX55002
Anti-Aurora A (phospho Thr288) antibody used in IHC (Paraffin sections) (IHC-P). GTX55002

Cat. No. GTX55002

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Aurora A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Aurora A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887910

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase A , AIK , ARK1 , AURA , BTAK , PPP1R47 , STK15 , STK6 , STK7

Background

The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Aurora A (phospho Thr288) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55002

GTX55002 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX55002 Aurora A (phospho Thr288) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Aurora A (phospho Thr288) antibody used in Western Blot (WB). GTX55002

GTX55002 WB Image

WB analysis of DLD (A), mouse brain (B), rat brain (C) whole cell lysates using GTX55002 Aurora A (phospho Thr288) antibody.

Anti-Aurora A (phospho Thr288) antibody used in IHC (Paraffin sections) (IHC-P). GTX55002

GTX55002 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX55002 Aurora A (phospho Thr288) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Wang JX et al. J Cell Physiol 2020; Aurora kinase inhibitor restrains STAT5-activated leukemic cell proliferation by inducing mitochondrial impairment.
Application : WB
Reactivity : Human
Xie Y et al. Gastroenterology 2017; 153 (5) : 1429-1443.e5 Inhibition of Aurora Kinase A Induces Necroptosis in?Pancreatic?Carcinoma.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399