APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1ug/ml |
| 1-2ug/ml |
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
Recombinant human Aurora B protein fragment (aa89-251) (exact sequence is proprietary)
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Aurora Kinase B , Aik2 , Aim-1 , Aim1 , Ark-2 , Ark2 , Aurb , Ipl1 , Ppp1R48 , Stk-1 , Stk12 , Stk5 , Aurkb-Sv1 , Aurkb-Sv2 , Aurkb
Background
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
![Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419](/upload/website/prouct_img/middle/GTX34419/GTX34419_20170712_WB_18121410_420.jpg "Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419")
|
GTX34419 WB Image
Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).
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REFERENCE
There are currently no references for Aurora B antibody [AURKB/1521] (GTX34419). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Aurora B antibody [AURKB/1521] (GTX34419). Be the first to share your experience with this product.
![Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419](/upload/website/prouct_img/small/GTX34419/GTX34419_20170712_WB_18121410_420.jpg "Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419")
![Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419](/upload/website/prouct_img/normal/GTX34419/GTX34419_20170712_WB_18121410_420.jpg "Anti-Aurora B antibody [AURKB/1521] used in Western Blot (WB). GTX34419")
