APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from hmuan Aurora B
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
aurora kinase B , AIK2 , AIM-1 , AIM1 , ARK-2 , ARK2 , AurB , IPL1 , PPP1R48 , STK-1 , STK12 , STK5 , aurkb-sv1 , aurkb-sv2
Cellular Localization
Nucleus
Background
This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis throμgh association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/templates/default/js/lightbox/images/loading.gif "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
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GTX00916 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody [GT1146] (GTX00916) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00916 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody [GT1146] (GTX00916) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX00916 WB Image
WB analysis of various samples using GTX00916 Aurora B antibody [GT1146].
Dilution : 1:1000
Loading : 25μg
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GTX00916 ICC/IF Image
ICC/IF analysis of C6 cells using GTX00916 Aurora B antibody [GT1146].
Red: Primary antibody
Blue: DAPI
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REFERENCE
There are currently no references for Aurora B antibody [GT1146] (GTX00916). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Aurora B antibody [GT1146] (GTX00916). Be the first to share your experience with this product.
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/small/GTX00916/GTX00916_40000000018_20200306_WB_treatment_Nocodazole_w_23053121_609.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/small/GTX00916/GTX00916_40000000018_20200306_WB_B_w_23053121_440.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/small/GTX00916/GTX00916_20200327_WB_1_w_23053121_596.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00916](/upload/website/prouct_img/small/GTX00916/GTX00916_20191101_AP_002_46_w_23053121_171.webp "Anti-Aurora B antibody [GT1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/normal/GTX00916/GTX00916_40000000018_20200306_WB_treatment_Nocodazole_w_23053121_609.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/normal/GTX00916/GTX00916_40000000018_20200306_WB_B_w_23053121_440.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916](/upload/website/prouct_img/normal/GTX00916/GTX00916_20200327_WB_1_w_23053121_596.webp "Anti-Aurora B antibody [GT1146] used in Western Blot (WB). GTX00916")
![Anti-Aurora B antibody [GT1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00916](/upload/website/prouct_img/normal/GTX00916/GTX00916_20191101_AP_002_46_w_23053121_171.webp "Anti-Aurora B antibody [GT1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00916")