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Aurora B antibody

Anti-Aurora B antibody used in Western Blot (WB). GTX130211
Anti-Aurora B antibody used in Western Blot (WB). GTX130211

Cat No. GTX130211

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

HeLa , HeLa (100 ng/ml Nocodazole, 24hr) , 293T transfected with Aurora B

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Aurora B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886207

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aurora kinase B , AIK2 , AIM-1 , AIM1 , ARK-2 , ARK2 , AurB , IPL1 , PPP1R48 , STK-1 , STK12 , STK5 , aurkb-sv1 , aurkb-sv2

Cellular Localization

Nucleus , Centromere

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Aurora B antibody used in Western Blot (WB). GTX130211

GTX130211 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX130211) diluted at 1:1000.

Anti-Aurora B antibody used in Western Blot (WB). GTX130211

GTX130211 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX130211) diluted at 1:5000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169