Aurora B antibody

Name: Aurora B antibody
Brand: GeneTex
SKU: GTX132702
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Aurora B antibody used in Western Blot (WB). GTX132702

Anti-Aurora B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132702

Cat. No. GTX132702

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX132702 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Myc-tagged AurkB-transfected 293T , HeLa (100 ng/ml Nocodazole treatment for 24hr)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Aurora B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886717

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase B , AIK2 , AIM-1 , AIM1 , ARK-2 , ARK2 , AurB , IPL1 , PPP1R48 , STK-1 , STK12 , STK5 , aurkb-sv1 , aurkb-sv2

Cellular Localization

Nucleus

Background

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Gene ID: 9212 AURKB
UniProt: Q96GD4 AURKB

Research Area

DATA IMAGES

GTX132702 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX132702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132702 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Aurora B antibody (GTX132702) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX132702 ICC/IF Image

Aurora B antibody detects Aurora B protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Aurora B stained by Aurora B antibody (GTX132702) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Liu F et al. Adv Sci (Weinh) 2022; 9 (22) : e2104823 A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells.

Application : WB

Reactivity : Human

Federico Gulluni et al. Science 2021; 374 (6573) : eabk0410 PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Application : ICC/IF

Reactivity : Human, Zebrafish

Ailin Chen et al. Cancer Commun (Lond) 2021; 41 (2) : 121-139 CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer.

Application : WB, ICC/IF

Reactivity : Human

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