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Aurora B (phospho Thr232) antibody

Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607
Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607
Anti-Aurora B (phospho Thr232) antibody used in Western Blot (WB). GTX85607
Anti-Aurora B (phospho Thr232) antibody used in Western Blot (WB). GTX85607
Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607

Cat. No. GTX85607

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, Dot, ELISA, Multiplexing

Reactivity

 Human, Monkey
Package
50 μg ($399)
See all Aurora B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
Dot Assay dependent
ELISA 1:10000-1:30000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Product Note

Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein.  Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_10722499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aurora kinase B , AIK2 , AIM-1 , AIM1 , ARK-2 , ARK2 , AurB , IPL1 , PPP1R48 , STK-1 , STK12 , STK5 , aurkb-sv1 , aurkb-sv2

Cellular Localization

Nucleus

Background

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607

GTX85607 IHC-P Image

IHC-P analysis of human intestine tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody.
Antigen retrieval : not performed.
Dilution : 10 μg/mL

Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607

GTX85607 IHC-P Image

IHC-P analysis of human placenta tissue (pH 9) using GTX85607 Aurora B (phospho Thr232) antibody.
Antigen retrieval : not performed.
Dilution : 10 μg/mL

Anti-Aurora B (phospho Thr232) antibody used in Western Blot (WB). GTX85607

GTX85607 WB Image

WB analysis of Nocodazole-treated Cos-7 whole cell lysate using GTX85607 Aurora B (phospho Thr232) antibody prior incubated with (Lane 2) or without (Lane 1) blocking peptide.
Dilution : 1:500

Anti-Aurora B (phospho Thr232) antibody used in Western Blot (WB). GTX85607

GTX85607 WB Image

Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500.
Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs)
Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen

Anti-Aurora B (phospho Thr232) antibody used in IHC (Paraffin sections) (IHC-P). GTX85607

GTX85607 IHC-P Image

Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody (GTX85607) at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.

REFERENCE

Application Reference

Jagtap AD et al. Eur J Med Chem 2014; 85C : 268-288 Novel acylureidoindolin-2-one derivatives as dual Aurora B/FLT3 inhibitors for the treatment of acute myeloid leukemia.
Application : Dot
Reactivity : Human
Wang HC et al. Eur J Med Chem 2014; 84C : 312-334 Bioisosteric replacement of an acylureido moiety attached to an indolin-2-one scaffold with a malonamido or a 2/4-pyridinoylamido moiety produces a selectively potent Aurora-B inhibitor.
Application : Dot
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399