GeneTex
United States (US)

Aurora C antibody, N-term

GTX88135_2479_WB_18122102_830.jpg

Cat No. GTX88135

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88135-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform 1 (NP_001015878.1) only.
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PRAVVQLGKAQP-C, from the N Terminus of the protein sequence according to NP_001015878.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Aurora Kinase C,Aie2,Aik3,Ark3,Aurc,Hel-S-90,Spgf5,Stk13,Aurora-C,Aurkc

Cellular Localization

Localized to centrosome from anaphase to cytokinesis.

Background

This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88135_2479_WB_18122102_830.jpg

GTX88135 WB Image

WB analysis of human testis lysate using GTX88135 Aurora C antibody, N-term.
Dilution : 1.5µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329