GeneTex
United States (US)

Autotaxin antibody

GTX106209_41759_20170615_WB_M_R_19011506_326.jpg
GTX106209_39764_IFA_19011506_158.jpg
GTX106209_42956_20180720_WB_cerebrospinalfluid_19011506_902.jpg

Cat No. GTX106209

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

human cerebrospinal fluid , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Autotaxin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2,Atx,Atx-X,Autotaxin,Lysopld,Npp2,Pd-Ialpha,Pdnp2,Enpp2

Cellular Localization

Secreted

Background

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106209_41759_20170615_WB_M_R_19011506_326.jpg

GTX106209 WB Image

Various tissue extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106209_39764_IFA_19011506_158.jpg

GTX106209 ICC/IF Image

Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Autotaxin protein stained by Autotaxin antibody (GTX106209) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106209_42956_20180720_WB_cerebrospinalfluid_19011506_902.jpg

GTX106209 WB Image

Human Cerebrospinal Fluid (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169