Human Cerebrospinal Fluid (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody (GTX106209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Autotaxin protein stained by Autotaxin antibody (GTX106209) diluted at 1:500.
Blue: Hoechst 33342 staining.

The data was published in the journal Sci Rep in 2020.PMID: 32111938