Axin 2 antibody [N2C2], Internal

Name: Axin 2 antibody [N2C2], Internal
Brand: GeneTex
SKU: GTX105442
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Axin 2 antibody [N2C2], Internal used in Western Blot (WB). GTX105442

Anti-Axin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105442

Cat. No. GTX105442

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Axin 2 products

View product citations for antibody GTX105442 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Axin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036304

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

axin 2 , AXIL , ODCRCS

Cellular Localization

Cytoplasm

Background

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Database

Gene ID: 8313 AXIN2
UniProt: Q9Y2T1 AXIN2

Research Area

DATA IMAGES

GTX105442 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Axin 2 antibody [N2C2], Internal (GTX105442) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX105442 IHC-P Image

axin 2 antibody [N2C2], Internal detects axin 2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
axin 2 antibody [N2C2], Internal (GTX105442) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105442 IHC-P Image

axin 2 antibody [N2C2], Internal detects axin 2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
axin 2 antibody [N2C2], Internal (GTX105442) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hsu TH et al. Cell Death Differ 2014; Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells.

Application : WB

Reactivity : Human

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