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Axin 2 antibody [N2C2], Internal

Anti-Axin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105442
Anti-Axin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105442

Cat No. GTX105442

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Axin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

axin 2 , AXIL , ODCRCS

Cellular Localization

Cytoplasm

Background

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Axin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105442

GTX105442 IHC-P Image

axin 2 antibody [N2C2], Internal detects axin 2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
axin 2 antibody [N2C2], Internal (GTX105442) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Axin 2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105442

GTX105442 IHC-P Image

axin 2 antibody [N2C2], Internal detects axin 2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
axin 2 antibody [N2C2], Internal (GTX105442) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Axin 2 antibody [N2C2], Internal Cat No. GTX105442

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 30-Mar-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT29 cells
Amount used : 35μg
Blocking : 10% skin milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169