GeneTex
United States (US)

Axin 2 antibody

GTX31822_2975_WB_20180221_18121410_449.jpg
GTX31822_1460_IHC-P_20180221_18121410_768.jpg
GTX31822_358_IHC-P_20180221_18121410_110.jpg
GTX31822_1459_IHC-P_20180221_18121410_432.jpg

Cat No. GTX31822

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Mouse Lung Tissue Lysate(GTX27933)

Specificity/Sensitivity

AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ZMYM3 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ZMYM3.The immunogen is located within amino acids 780 - 830 of ZMYM3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Axin 2,Axil,Odcrcs,Axin2

Cellular Localization

Cytoplasm

Background

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31822_2975_WB_20180221_18121410_449.jpg

GTX31822 WB Image

WB analysis of mouse lung tissue lysate using GTX31822 Axin 2 antibody.
Working concentration : 1 μg/ml

GTX31822_1460_IHC-P_20180221_18121410_768.jpg

GTX31822 IHC-P Image

IHC-P analysis of rat lung tissue using GTX31822 Axin 2 antibody.
Working concentration : 5 μg/ml

GTX31822_358_IHC-P_20180221_18121410_110.jpg

GTX31822 IHC-P Image

IHC-P analysis of rat lung tissue using GTX31822 Axin 2 antibody.
Working concentration : 20 μg/ml

GTX31822_1459_IHC-P_20180221_18121410_432.jpg

GTX31822 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX31822 Axin 2 antibody.
Working concentration : 5 μg/ml