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B-Raf (K601E Mutant) antibody

Anti-B-Raf (K601E Mutant) antibody used in Western Blot (WB). GTX135414
Anti-B-Raf (K601E Mutant) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135414

Cat No. GTX135414

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant K601E of human B-Raf. The exact sequence is proprietary.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2909852

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-B-Raf (K601E Mutant) antibody used in Western Blot (WB). GTX135414

GTX135414 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf (K601E Mutant) antibody (GTX135414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf (K601E Mutant) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135414

GTX135414 ICC/IF Image

B-Raf (K601E Mutant) antibody detects B-Raf (K601E Mutant) protein by immunofluorescent analysis.
Sample: WT transfected and mutant transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: B-Raf (K601E Mutant) stained by B-Raf (K601E Mutant) antibody (GTX135414) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169