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B-Raf antibody [1D2]

Overexpression
Anti-B-Raf antibody [1D2] used in Western Blot (WB). GTX84807
Anti-B-Raf antibody [1D2] used in Flow cytometry (FACS). GTX84807
Anti-B-Raf antibody [1D2] used in Flow cytometry (FACS). GTX84807
Anti-B-Raf antibody [1D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84807
Anti-B-Raf antibody [1D2] used in Western Blot (WB). GTX84807

Cat. No. GTX84807

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [1D2] used in Western Blot (WB). GTX84807

GTX84807 WB Image

Overexpression

WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84807 B-Raf antibody [1D2].
Loading : 5 ug per lane

Anti-B-Raf antibody [1D2] used in Flow cytometry (FACS). GTX84807

GTX84807 FACS Image

FACS analysis of Jurkat cells using GTX84807 B-Raf antibody [1D2].
Red : Primary antibody
Blue : Negative control antibody

Anti-B-Raf antibody [1D2] used in Flow cytometry (FACS). GTX84807

GTX84807 FACS Image

FACS analysis of HeLa cells using GTX84807 B-Raf antibody [1D2].
Red : Primary antibody
Blue : Negative control antibody

Anti-B-Raf antibody [1D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84807

GTX84807 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with B-Raf plasmid using GTX84807 B-Raf antibody [1D2].

Anti-B-Raf antibody [1D2] used in Western Blot (WB). GTX84807

GTX84807 WB Image

WB analysis of SVT2 cell lysate using GTX84807 B-Raf antibody [1D2].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519