B-Raf antibody [4B2]

Cat. No. GTX84814
Cat. No.  GTX84814
Overexpression
Anti-B-Raf antibody [4B2] used in Immunoprecipitation (IP). GTX84814
GTX84814 IP Image

IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84814 B-Raf antibody [4B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

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Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
GTX84814 WB Image
Overexpression

WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84814 B-Raf antibody [4B2].
Loading : 5 ug per lane

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Anti-B-Raf antibody [4B2] used in ELISA (ELISA). GTX84814
GTX84814 ELISA Image

Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.

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Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
GTX84814 WB Image

WB analysis of various cell lines using GTX84814 B-Raf antibody [4B2].
Loading : 35 ug per lane

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Anti-B-Raf antibody [4B2] used in Immunoprecipitation (IP). GTX84814
Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
Anti-B-Raf antibody [4B2] used in ELISA (ELISA). GTX84814
Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    4B2
  • Isotype
    IgG2a
  • Applications
    WB IP ELISA
  • Reactivity
    Human, Monkey
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