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B-Raf antibody [4B2]

Overexpression
Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
Anti-B-Raf antibody [4B2] used in ELISA (ELISA). GTX84814
Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814
Anti-B-Raf antibody [4B2] used in Immunoprecipitation (IP). GTX84814

Cat. No. GTX84814

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B2

Isotype

 IgG2a

Application

 WB, IP, ELISA

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human BRAF expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814

GTX84814 WB Image

WB analysis of various cell lines using GTX84814 B-Raf antibody [4B2].
Loading : 35 ug per lane

Anti-B-Raf antibody [4B2] used in ELISA (ELISA). GTX84814

GTX84814 ELISA Image

Sandwich ELISA analysis of BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using GTX84820 B-Raf antibody [4A5] (capture antibody) at 5ug/ml and HRP conjugated GTX84814 B-Raf antibody [4B2] (detection antibody) at 0.03ug/ml.

Anti-B-Raf antibody [4B2] used in Western Blot (WB). GTX84814

GTX84814 WB Image

Overexpression

WB analysis of HEK293T cells transfected with B-Raf plasmid (Right) or empty vector (Left) for 48 hrs using GTX84814 B-Raf antibody [4B2].
Loading : 5 ug per lane

Anti-B-Raf antibody [4B2] used in Immunoprecipitation (IP). GTX84814

GTX84814 IP Image

IP analysis of DDDDK tagged B-Raf overexpressed HEK293T lysate using GTX84814 B-Raf antibody [4B2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519