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B-Raf antibody [HL1606]

Recombinant antibody KO/KD Overexpression
Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085
Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085
Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085
Anti-B-Raf antibody [HL1606] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637085
Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085
Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085
Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085
Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085
Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085

Cat. No. GTX637085

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1606

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085

GTX637085 WB Image

KO/KD

Wild-type (WT) and BRAF knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085

GTX637085 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085

GTX637085 IHC-P Image

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [HL1606] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637085

GTX637085 ICC/IF Image

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085

GTX637085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085

GTX637085 IHC-P Image

B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085

GTX637085 IHC-P Image

B-Raf antibody [HL1606] detects B-Raf protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [HL1606] used in IHC (Paraffin sections) (IHC-P). GTX637085

GTX637085 IHC-P Image

B-Raf antibody [HL1606] detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [HL1606] (GTX637085) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [HL1606] used in Western Blot (WB). GTX637085

GTX637085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [HL1606] (GTX637085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations