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B-Raf antibody [N2C1], Internal

Overexpression
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

Cat No. GTX100913

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all B-Raf products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

PC-12 , human BRAF-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036363

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
7.5 % SDS-PAGE
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Overexpression

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.

Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913

GTX100913 IP Image

B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913) 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tsuji S et al. Oncol Lett 2021; 21 (2) : 113 Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.
Application : WB
Reactivity : Human
Tateishi K et al. Acta Neuropathol Commun 2020; 8 (1) : 139 BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young.
Application : WB
Reactivity : Human
Sase H et al. Mol Cancer Ther 2018; (Epub) Acquired JHDM1D-BRAF fusion confers resistance to FGFR inhibition in FGFR2- amplified gastric cancer.
Application : WB
Reactivity : Human
Rubin B et al. Cancer Invest 2015; (Epub) Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169