APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.25 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human B-Raf. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887352
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1
Cellular Localization
Nucleus,Cytoplasm,Cell membrane
Background
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX134824 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody (GTX134824) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134824 IHC-P Image
B-Raf antibody detects B-Raf protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.B-Raf stained by B-Raf antibody (GTX134824) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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