GeneTex
  • Country / Location Selection

United States (US)

B-Raf antibody

Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881
Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881
Anti-B-Raf antibody used in Western Blot (WB). GTX31881

Cat No. GTX31881

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918) , Human Small Intestine Tissue Slide

Specificity/Sensitivity

This antibody will not cross-react with C-raf.

Predict Reactivity

Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf.The immunogen is located within amino acids 330 - 380 of B-raf.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881

GTX31881 IHC-P Image

IHC-P analysis of formaldehyde fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL

Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881

GTX31881 IHC-P Image

IHC-P analysis of 4% PFA-fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Dilution : 20μg/mL

Anti-B-Raf antibody used in Western Blot (WB). GTX31881

GTX31881 WB Image

WB analysis of various sample lysates using GTX31881 B-Raf antibody.
Loading : 15μg of lysates per lane
Dilution : 0.5 μg/ml

REFERENCE

There are currently no references for B-Raf antibody (GTX31881). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for B-Raf antibody (GTX31881). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339