APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-2 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Lysate(GTX27918) , Human Small Intestine Tissue Slide
Specificity/Sensitivity
This antibody will not cross-react with C-raf.
Predict Reactivity
Rat, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS with 0.02% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf.The immunogen is located within amino acids 330 - 380 of B-raf.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1
Cellular Localization
Nucleus,Cytoplasm,Cell membrane
Background
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX31881 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL
|
|
GTX31881 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Dilution : 20μg/mL
|
|
GTX31881 WB Image
WB analysis of various sample lysates using GTX31881 B-Raf antibody.
Loading : 15μg of lysates per lane
Dilution : 0.5 μg/ml
|
REFERENCE
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REVIEW
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