GeneTex
United States (US)

B-Raf antibody

Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881
Anti-B-Raf antibody used in Western Blot (WB). GTX31881
Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881

Cat No. GTX31881

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918) , Human Small Intestine Tissue Slide

Specificity/Sensitivity

At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf.The immunogen is located within amino acids 330 - 380 of B-raf.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B-Raf Proto-Oncogene, Serine/Threonine Kinase,B-Raf1,B-Raf,Braf1,Ns7,Rafb1,Braf

Background

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881

GTX31881 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31881 B-Raf antibody.
Working concentration : 20 μg/ml

Anti-B-Raf antibody used in Western Blot (WB). GTX31881

GTX31881 WB Image

WB analysis of human brain tissue lysate using GTX31881 B-Raf antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-B-Raf antibody used in IHC (Paraffin sections) (IHC-P). GTX31881

GTX31881 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX31881 B-Raf antibody.
Working concentration : 2.5 μg/ml

Package List Price ($)
$ 329