APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Lysate(GTX27918) , Human Small Intestine Tissue Slide
Product Note
This antibody will not cross-react with C-raf.
Predict Reactivity
Rat, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf.The immunogen is located within amino acids 330 - 380 of B-raf.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1
Cellular Localization
Nucleus,Cytoplasm,Cell membrane
Background
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31881 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Dilution : 20μg/mL
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GTX31881 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Small Intestine Tissue using GTX31881 B-Raf antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL
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GTX31881 WB Image
WB analysis of various sample lysates using GTX31881 B-Raf antibody.
Loading : 15μg of lysates per lane
Dilution : 0.5 μg/ml
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REFERENCE
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REVIEW
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