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B-Raf antibody

Anti-B-Raf antibody used in Western Blot (WB). GTX85102
Anti-B-Raf antibody used in Western Blot (WB). GTX85102

Cat No. GTX85102

Host

Chicken

Clonality

Polyclonal

Isotype

IgY

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

B-raf antibody was raised against an 18 amino acid synthetic peptide near the center of human B-raf.The immunogen is located within amino acids 330 - 380 of B-raf.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Database

Research Area

DATA IMAGES

Anti-B-Raf antibody used in Western Blot (WB). GTX85102

GTX85102 WB Image

WB analysis of various samples using GTX85102 B-Raf antibody.
Dilution : 0.5μg/mL
Loading : 15μg of lysates per lane

Anti-B-Raf antibody used in Western Blot (WB). GTX85102

GTX85102 WB Image

WB analysis of various samples using GTX85102 B-Raf antibody.
Dilution : 0.5μg/mL
Loading : 15μg of lysates per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399