APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88996-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SKGKGNANLEYK, from the internal region of the protein sequence according to NP_078902.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
V-Set Domain Containing T Cell Activation Inhibitor 1,B7-H4,B7H4,B7S1,B7X,B7H.5,Pro1291,Vctn1,Vtcn1
Cellular Localization
Expressed at the cell surface. A soluble form has also been detected.
Background
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Database
DATA IMAGES
|
GTX88996 WB Image
WB analysis of human pancreas lysate using GTX88996 B7-H4 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer
|
REFERENCE
There are currently no references for B7-H4 antibody, Internal (GTX88996). Be the first to share your publications with this product.
REVIEW
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