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B7-H4 antibody

Anti-B7-H4 antibody used in Western Blot (WB). GTX03785
Anti-B7-H4 antibody used in Flow cytometry (FACS). GTX03785
Anti-B7-H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03785
Anti-B7-H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03785

Cat. No. GTX03785

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($429)
See all B7-H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
ICC/IF 2μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x106 cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human B7H4/VTCN1 recombinant protein (Position: T57-A258).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

V-set domain containing T cell activation inhibitor 1 , B7-H4 , B7H4 , B7S1 , B7X , B7h.5 , PRO1291 , VCTN1

Cellular Localization

Cell membrane

Background

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-B7-H4 antibody used in Western Blot (WB). GTX03785

GTX03785 WB Image

WB analysis of various lysates using GTX03785 B7-H4 antibody.
Lane 1 : U2OS cells
Lane 2 : Mouse thymus issue
Dilution : 0.5 μg/mL
Loading : 50μg per lane

Anti-B7-H4 antibody used in Flow cytometry (FACS). GTX03785

GTX03785 FACS Image

FACS analysis of U2OS cells using GTX03785 B7-H4 antibody.
Antibody amount : 1μg/1x106 cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-B7-H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03785

GTX03785 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX03785 B7-H4 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 1μg/ml

Anti-B7-H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03785

GTX03785 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03785 B7-H4 antibody.
Antigen retireval : Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 429