ICC/IF analysis of U2OS cells using GTX03785 B7-H4 antibody.
Antigen retireval : Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins.
Dilution : 2μg/mL

IHC-P analysis of human mammary cancer tissue using GTX03785 B7-H4 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 1μg/ml

FACS analysis of U2OS cells using GTX03785 B7-H4 antibody.
Antibody amount : 1μg/1x106 cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

WB analysis of various lysates using GTX03785 B7-H4 antibody.
Lane 1 : U2OS cells
Lane 2 : Mouse thymus issue
Dilution : 0.5 μg/mL
Loading : 50μg per lane