GeneTex
United States (US)

B9D1 antibody

Anti-B9D1 antibody used in Western Blot (WB). GTX32055
Anti-B9D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32055
Anti-B9D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32055

Cat No. GTX32055

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

Specificity/Sensitivity

At least two isoforms of B9D1 are known to exist; this antibody will only recognize the longest isoform. B9D1 antibody is predicted to not cross-react with other DNAJC family members.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

B9D1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human B9D1.The immunogen is located within amino acids 130 - 180 of B9D1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B9 Domain Containing 1,B9,Eppb9,Jbts27,Mks9,Mksr1,B9D1

Background

This gene encodes a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. Meckel syndrome has been associated with at least six different genes. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Three alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
Anti-B9D1 antibody used in Western Blot (WB). GTX32055

GTX32055 WB Image

WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32055 B9D1 antibody.
Working concentration : 1 μg/ml

Anti-B9D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32055

GTX32055 ICC/IF Image

ICC/IF analysis of 293 cells using GTX32055 B9D1 antibody.
Working concentration : 5 μg/ml

Anti-B9D1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32055

GTX32055 ICC/IF Image

ICC/IF analysis of 293 cells using GTX32055 B9D1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329