GeneTex
United States (US)

BACE1 antibody [N1C1]

GTX113319_40394_IHC_M_18121410_895.jpg
GTX113319_40394_IHC_18121410_240.jpg
GTX113319_40394_20171215_WB_B_18121410_256.jpg

Cat No. GTX113319

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

BACE1-transfected 293T

Predict Reactivity

Rat, Bovine, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BACE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ASP2 antibody, BACE antibody, FLJ90568 antibody, HSPC104 antibody, KIAA1149 antibody, BACE1 antibody, beta-site amyloid beta A4 precursor protein-cleaving enzyme antibody, beta-secretase 1 precursor variant 1 antibody, membrane-associated aspartic protease 2 antibody, aspartyl protease 2 antibody, transmembrane aspartic proteinase Asp2 antibody, APP beta-secretase antibody, beta-secretase 1 antibody, asp 2 antibody, beta-site APP cleaving enzyme 1 antibody, memapsin-2 antibody, beta-site APP-cleaving enzyme 1 antibody

Cellular Localization

Membrane

Background

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX113319_40394_IHC_M_18121410_895.jpg

GTX113319 IHC-P Image

BACE1 antibody [N1C1] detects BACE1 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
BACE1 antibody [N1C1] (GTX113319) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113319_40394_IHC_18121410_240.jpg

GTX113319 IHC-P Image

BACE1 antibody [N1C1] detects BACE1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
BACE1 antibody [N1C1] (GTX113319) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113319_40394_20171215_WB_B_18121410_256.jpg

GTX113319 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BACE1 antibody [N1C1] (GTX113319) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf