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BACE1 antibody [N1C2]

Anti-BACE1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103757
Anti-BACE1 antibody [N1C2] used in Western Blot (WB). GTX103757
Anti-BACE1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103757

Cat. No. GTX103757

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all BACE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Human, Zebrafish, Bovine, Guinea pig, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human BACE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165083

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-secretase 1 , ASP2 , BACE , HSPC104

Cellular Localization

Cell membrane,Endoplasmic reticulum,Endosome,Lysosome

Background

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-BACE1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103757

GTX103757 IHC-P Image

BACE1 antibody [N1C2] detects BACE1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
BACE1 stained by BACE1 antibody [N1C2] (GTX103757) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BACE1 antibody [N1C2] used in Western Blot (WB). GTX103757

GTX103757 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BACE1 antibody [N1C2] (GTX103757) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BACE1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103757

GTX103757 IHC-P Image

BACE1 antibody [N1C2] detects BACE1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
BACE1 stained by BACE1 antibody [N1C2] (GTX103757) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kumro J et al. bioRxiv 2024; α7 nicotinic acetylcholine receptors are necessary for basal forebrain activation to increase expression of the nerve growth factor receptor TrkA.
Kumro J et al. Cereb Cortex 2023; 33 (12) : 7627-7641 Chronic basal forebrain activation improves spatial memory, boosts neurotrophin receptor expression, and lowers BACE1 and Aβ42 levels in the cerebral cortex in mice.
Liu QS et al. Life Sci 2017; (Epub) Total flavonoid extract from Dracoephalum moldavica L. attenuates β-amyloid-induced toxicity through anti-amyloidogenesic and neurotrophic pathways.
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169
Currently in production