GeneTex
United States (US)

BACE2 antibody [C1C3]

GTX113951_40485_IHC_R_18121410_501.jpg
GTX113951_40485_IFA_18121410_762.jpg
GTX113951_40485_WB_18121410_328.jpg
GTX113951_40485_IHC_18121410_677.jpg
GTX113951_40485_20160106_IHC-P_R_18121410_998.jpg

Cat No. GTX113951

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.83mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BACE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AEPLC antibody, ALP56 antibody, ASP1 antibody, ASP21 antibody, BAE2 antibody, CEAP1 antibody, DRAP antibody, BACE2 antibody, Down syndrome region aspartic protease antibody, beta-site amyloid precursor protein cleaving enzyme 2 antibody, beta secretase 2 antibody, memapsin-1 antibody, beta-site APP cleaving enzyme 2 antibody, 56 kDa aspartic-like protease antibody, theta-secretase antibody, aspartic-like protease 56 kDa antibody, down region aspartic protease antibody, aspartyl protease 1 antibody, beta-site amyloid beta A4 precursor protein-cleaving enzyme 2 antibody, asp 1 antibody, membrane-associated aspartic protease 1 antibody, beta-secretase 2 antibody, transmembrane aspartic proteinase Asp1 antibody, beta-site APP-cleaving enzyme 2 antibody

Cellular Localization

Membrane

Background

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease and a frequent complication of Down syndrome. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein by 2 proteases, one of which is the protein encoded by this gene. This gene localizes to the 'Down critical region' of chromosome 21. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX113951_40485_IHC_R_18121410_501.jpg

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
BACE2 antibody [C1C3] (GTX113951) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113951_40485_IFA_18121410_762.jpg

GTX113951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BACE2(GTX113951) antibody at 1:500 dilution.

GTX113951_40485_WB_18121410_328.jpg

GTX113951 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX113951 diluted at 1:1000

GTX113951_40485_IHC_18121410_677.jpg

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BACE2 antibody [C1C3] (GTX113951) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113951_40485_20160106_IHC-P_R_18121410_998.jpg

GTX113951 IHC-P Image

BACE2 antibody [C1C3] detects BACE2 protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
BACE2 antibody [C1C3] (GTX113951) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf