GeneTex

  • Country / Location Selection

United States (US)

BAF53B antibody

Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122920
Anti-BAF53B antibody used in Western Blot (WB). GTX122920
Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122920

Cat. No. GTX122920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all BAF53B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

SK-N-SH

Predict Reactivity

Mouse, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAF53B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin like 6B , ACTL6 , BAF53B , arpNalpha

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122920

GTX122920 ICC/IF Image

BAF53B antibody detects BAF53B protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAF53B stained by BAF53B antibody (GTX122920) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BAF53B antibody used in Western Blot (WB). GTX122920

GTX122920 WB Image

Sample (30 ug of whole cell lysate)
A: SK-N-SH
10% SDS PAGE
GTX122920 diluted at 1:1000

Anti-BAF53B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122920

GTX122920 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Actin like 6B(GTX122920) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

There are currently no references for BAF53B antibody (GTX122920). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BAF53B antibody (GTX122920). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169