APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-0.6μg/ml |
| 3-5μg/ml |
|
Note :
IHC-P
Human Prostate shows nuclear staining in secretory epithelial cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89755-PEP).
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 , BAF57 , CSS5
Cellular Localization
Nucleus
Background
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89755 WB Image
WB analysis of (A) HEK293 overexpressing BAF57 (mock transfection in first lane) (B) Jurkat lysate (35μg) using GTX89755 BAF57 antibody, C-term.
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GTX89755 IHC-P Image
IHC-P analysis of human prostate using GTX89755 BAF57 antibody, C-term.
It shows nuclear staining in secretory epithelial cells.
Dilution : 3-5μg/ml
|
REFERENCE
There are currently no references for BAF57 antibody, C-term (GTX89755). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BAF57 antibody, C-term (GTX89755). Be the first to share your experience with this product.
