GeneTex
United States (US)

BAF57 antibody [C3], C-term

GTX106345_WB_19011506_942.jpg
GTX106345_39757_IFA_19011506_745.jpg
GTX106345_39757_IHC_19011506_498.jpg

Cat No. GTX106345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BAF57. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BAF57 antibody, FLJ35648 antibody, SMARCE1 antibody, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1 antibody, BRG1-associated factor 57 antibody, chromatin remodeling complex BRG1-associated factor 57 antibody, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody, "SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 antibody"

Cellular Localization

Nucleus

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq]

Database

DATA IMAGES
GTX106345_WB_19011506_942.jpg

GTX106345 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX106345 diluted at 1:3000

GTX106345_39757_IFA_19011506_745.jpg

GTX106345 ICC/IF Image

BAF57 antibody [C3], C-term detects BAF57 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAF57 protein stained by BAF57 antibody [C3], C-term (GTX106345) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX106345_39757_IHC_19011506_498.jpg

GTX106345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung, using BAF57(GTX106345) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169