APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji
Predict Reactivity
Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.04 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BAF57. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951941
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 , BAF57 , CSS5
Cellular Localization
Nucleus
Background
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345](/templates/default/js/lightbox/images/loading.gif "Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345")
|
GTX106345 WB Image
Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX106345 diluted at 1:3000
|
|
|
GTX106345 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung, using BAF57(GTX106345) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX106345 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAF57 antibody [C3], C-term (GTX106345) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX106345 ICC/IF Image
BAF57 antibody [C3], C-term detects BAF57 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAF57 protein stained by BAF57 antibody [C3], C-term (GTX106345) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
REFERENCE
There are currently no references for BAF57 antibody [C3], C-term (GTX106345). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BAF57 antibody [C3], C-term (GTX106345). Be the first to share your experience with this product.
![Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345](/upload/website/prouct_img/small/GTX106345/GTX106345_WB_w_23060120_243.webp "Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106345](/upload/website/prouct_img/small/GTX106345/GTX106345_39757_IHC_w_23060120_904.webp "Anti-BAF57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345](/upload/website/prouct_img/small/GTX106345/GTX106345_39757_20220304_WB_w_23060120_938.webp "Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106345](/upload/website/prouct_img/small/GTX106345/GTX106345_39757_IFA_w_23060120_153.webp "Anti-BAF57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345](/upload/website/prouct_img/normal/GTX106345/GTX106345_WB_w_23060120_243.webp "Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106345](/upload/website/prouct_img/normal/GTX106345/GTX106345_39757_IHC_w_23060120_904.webp "Anti-BAF57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345](/upload/website/prouct_img/normal/GTX106345/GTX106345_39757_20220304_WB_w_23060120_938.webp "Anti-BAF57 antibody [C3], C-term used in Western Blot (WB). GTX106345")
![Anti-BAF57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106345](/upload/website/prouct_img/normal/GTX106345/GTX106345_39757_IFA_w_23060120_153.webp "Anti-BAF57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106345")