GeneTex
United States (US)

BAF57 antibody

GTX114781_WB_18121410_445.jpg
GTX114781_40233_IFA_18121410_693.jpg
GTX114781_40233_IHC_18121410_705.jpg
GTX114781_40233_WB_M_18121410_710.jpg
GTX114781_40233_20150410_IFA_18121410_152.jpg

Cat No. GTX114781

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

IMR32 , Jurkat , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAF57. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily E, Member 1,Baf57,Css5,Smarce1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq]

Database

DATA IMAGES
GTX114781_WB_18121410_445.jpg

GTX114781 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
B: JurKat
10% SDS PAGE
GTX114781 diluted at 1:1000

GTX114781_40233_IFA_18121410_693.jpg

GTX114781 ICC/IF Image

BAF57 antibody detects SMARCE1 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMARCE1 protein stained by BAF57 antibody (GTX114781) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX114781_40233_IHC_18121410_705.jpg

GTX114781 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using BAF57(GTX114781) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114781_40233_WB_M_18121410_710.jpg

GTX114781 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX114781 diluted at 1:1000

GTX114781_40233_20150410_IFA_18121410_152.jpg

GTX114781 ICC/IF Image

BAF57 antibody detects BAF57 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAF57 protein stained by BAF57 antibody (GTX114781) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169