GeneTex
United States (US)

BAF57 antibody

KO/KD
GTX35217_20180822_WB_18121410_948.jpg
GTX35217_20180822_IP_18121410_284.jpg
GTX35217_20180822_ICCIF_18121410_797.jpg
GTX35217_20180822_WB_1_18121410_991.jpg

Cat No. GTX35217

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

47 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily E, Member 1,Baf57,Css5,Smarce1

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX35217_20180822_WB_18121410_948.jpg

GTX35217 WB Image

KO/KD

WB analysis of various samples using GTX35217 BAF57 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35217_20180822_IP_18121410_284.jpg

GTX35217 IP Image

KO/KD

IP analysis of Jurkat cell lysate using GTX35217 BAF57 antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:1000

GTX35217_20180822_ICCIF_18121410_797.jpg

GTX35217 ICC/IF Image

KO/KD

ICC/IF ananlysis of HeLa cells using GTX35217 BAF57 antibody.
Dilution : 1:100

GTX35217_20180822_WB_1_18121410_991.jpg

GTX35217 WB Image

KO/KD

WB analysis of wild type and BAF57 knockout cell lysate using GTX35217 BAF57 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329