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BAF57 antibody

KO/KD
Anti-BAF57 antibody used in Western Blot (WB). GTX35217
Anti-BAF57 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35217
Anti-BAF57 antibody used in Immunoprecipitation (IP). GTX35217
Anti-BAF57 antibody used in Western Blot (WB). GTX35217

Cat. No. GTX35217

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all BAF57 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 , BAF57 , CSS5

Cellular Localization

Nucleus

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BAF57 antibody used in Western Blot (WB). GTX35217

GTX35217 WB Image

WB analysis of various sample lysates using GTX35217 BAF57 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BAF57 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35217

GTX35217 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35217 BAF57 antibody.

Anti-BAF57 antibody used in Immunoprecipitation (IP). GTX35217

GTX35217 IP Image

IP analysis of Jurkat cell lysate using GTX35217 BAF57 antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:1000

Anti-BAF57 antibody used in Western Blot (WB). GTX35217

GTX35217 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35217 BAF57 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399