GeneTex
United States (US)

BAFF antibody [Buffy-1]

BAFF-antibody-Buffy-1_Flow-cytometryFACS_GTX16107-1_18121409_901.jpg
img25864_18121411_249.jpg

Cat No. GTX16107

Host Rat
Clonality Monoclonal
Clone Name Buffy-1
Isotype IgG2a
Application WB, ICC/IF, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent

Note :

FACS
HEK 293T cells (5 x 10?) were mock transfected or transfected with BAFF.

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Specificity/Sensitivity

Recognizes membrane-bound human BAFF. Detects endogenous protein by Flow Cytometry.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BAFF (monoclonal Buffy-1) antibody was raised against recombinant human soluble BAFF (aa. 83-285).

Purification

Purified IgG2a

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Superfamily Member 13B,Baff,Blys,Cd257,Dtl,Tall-1,Tall1,Thank,Tnfsf20,Tnlg7A,Ztnf4,Tnfsf13B

Cellular Localization

Type II membrane protein. Also exists as an extracellular soluble form.

Background

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL,THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.

Database

DATA IMAGES
BAFF-antibody-Buffy-1_Flow-cytometryFACS_GTX16107-1_18121409_901.jpg

GTX16107 FACS Image

It shows the flow cytometric profile of 293 cells stably transfected with a BAFF-expression plasmid (1). Control (2) are mock transfected 293 cells. Optimal concentration range is 0.5-5 µg/ml.

img25864_18121411_249.jpg

GTX16107 ICC/IF Image

BAFF antibody [Buffy-1]

Package List Price ($)
$ 329