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BAG2 antibody [AT29E9]

Anti-BAG2 antibody [AT29E9] used in Western Blot (WB). GTX57685
Anti-BAG2 antibody [AT29E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57685

Cat. No. GTX57685

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT29E9

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all BAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT29E9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BAG4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAG cochaperone 2 , BAG2 , BCL2 associated athanogene 2 , dJ417I1.2

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BAG2 antibody [AT29E9] used in Western Blot (WB). GTX57685

GTX57685 WB Image

WB analysis of various samples using GTX57685 BAG2 antibody.
Lane 1 : Jurkat whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-BAG2 antibody [AT29E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57685

GTX57685 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57685 BAG2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399