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BAG2 antibody, C-term

Anti-BAG2 antibody, C-term used in Western Blot (WB). GTX89538
Anti-BAG2 antibody, C-term used in Western Blot (WB). GTX89538

Cat. No. GTX89538

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Pig
Package
100 μg ($399)
See all BAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89538-PEP).

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CSKTLQQNAESRFN, from the C Terminus of the protein sequence according to NP_004273.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 2 , BAG-2 , dJ417I1.2

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BAG2 antibody, C-term used in Western Blot (WB). GTX89538

GTX89538 WB Image

WB analysis of HeLa lysate using GTX89538 BAG2 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-BAG2 antibody, C-term used in Western Blot (WB). GTX89538

GTX89538 WB Image

WB analysis of pig testis lysate using GTX89538 BAG2 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399