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BAG2 antibody

Anti-BAG2 antibody used in Western Blot (WB). GTX33033
Anti-BAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33033
Anti-BAG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX33033

Cat. No. GTX33033

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all BAG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 2 , BAG-2 , dJ417I1.2

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BAG2 antibody used in Western Blot (WB). GTX33033

GTX33033 WB Image

WB analysis of various sample lysates using GTX33033 BAG2 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BAG2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33033

GTX33033 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33033 BAG2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-BAG2 antibody used in IHC (Paraffin sections) (IHC-P). GTX33033

GTX33033 IHC-P Image

IHC-P analysis of human kidney tissue using GTX33033 BAG2 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399