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BAG3 antibody [N1C1]

Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343
Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343
Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343

Cat. No. GTX102343

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all BAG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

MCF-7 , rat heart

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAG3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724402

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 3 , BAG-3 , BIS , CAIR-1 , MFM6

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343

GTX102343 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX102343 diluted at 1:1000

Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343

GTX102343 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody [N1C1] (GTX102343) diluted at 1:500.

Anti-BAG3 antibody [N1C1] used in Western Blot (WB). GTX102343

GTX102343 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody [N1C1] (GTX102343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Salim C et al. J Orofac Orthop 2021; Role of chaperone-assisted selective autophagy (CASA) in mechanical stress protection of periodontal ligament cells.
Application : WB
Reactivity : Human
Li C et al. Front Oncol 2019; 9 : 225 BAG3 Suppresses Loading of Ago2 to IL6 mRNA in Pancreatic Ductal Adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169