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BAG3 antibody

Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777
Anti-BAG3 antibody used in Western Blot (WB). GTX02777
Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777
Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777

Cat. No. GTX02777

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BAG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 3 , BAG-3 , BIS , CAIR-1 , MFM6

Cellular Localization

Nucleus , Cytoplasm

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777

GTX02777 IHC-P Image

IHC-P analysis of human lung tissue using GTX02777 BAG3 antibody.
Dilution : 1:100

Anti-BAG3 antibody used in Western Blot (WB). GTX02777

GTX02777 WB Image

WB analysis of various sample lysates using GTX02777 BAG3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777

GTX02777 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX02777 BAG3 antibody.
Dilution : 1:100

Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX02777

GTX02777 IHC-P Image

IHC-P analysis of rat heart tissue using GTX02777 BAG3 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399