GeneTex
United States (US)

BAG3 antibody

GTX102396_39694_WB_M_heart_19011506_704.jpg
GTX102396_IHC_19011506_763.jpg
GTX102396_39694_WB_19011506_978.jpg

Cat No. GTX102396

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

K562 , mouse heart

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BAG3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BAG-3 antibody, BIS antibody, CAIR-1 antibody, MGC104307 antibody, BAG3 antibody, docking protein CAIR-1 antibody, bcl-2-binding protein Bis antibody, BAG family molecular chaperone regulator 3 antibody, bcl-2-associated athanogene 3 antibody, BAG-family molecular chaperone regulator-3 antibody, BCL2-binding athanogene 3 antibody, BCL2-associated athanogene 3 antibody

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102396_39694_WB_M_heart_19011506_704.jpg

GTX102396 WB Image

BAG3 antibody detects BAG3 protein by western blot analysis.
A. 50 µg mouse heart lysate/extract
7.5% SDS-PAGE
BAG3 antibody (GTX102396) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102396_IHC_19011506_763.jpg

GTX102396 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BAG3(GTX102396) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102396_39694_WB_19011506_978.jpg

GTX102396 WB Image

BAG3 antibody detects BAG3 protein by western blot analysis.
A. 30 µg K562 whole cell lysate/extract
7.5% SDS-PAGE
BAG3 antibody (GTX102396) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169