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BAG3 antibody

Anti-BAG3 antibody used in Western Blot (WB). GTX102396
Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX102396
Anti-BAG3 antibody used in Western Blot (WB). GTX102396

Cat No. GTX102396

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
See all BAG3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

K562 , mouse heart

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BAG3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL2 associated athanogene 3 , BAG-3 , BIS , CAIR-1 , MFM6

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BAG3 antibody used in Western Blot (WB). GTX102396

GTX102396 WB Image

BAG3 antibody detects BAG3 protein by western blot analysis.
A. 50 µg mouse heart lysate/extract
7.5% SDS-PAGE
BAG3 antibody (GTX102396) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-BAG3 antibody used in IHC (Paraffin sections) (IHC-P). GTX102396

GTX102396 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BAG3(GTX102396) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-BAG3 antibody used in Western Blot (WB). GTX102396

GTX102396 WB Image

BAG3 antibody detects BAG3 protein by western blot analysis.
A. 30 µg K562 whole cell lysate/extract
7.5% SDS-PAGE
BAG3 antibody (GTX102396) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Yuan Y et al. J Cell Mol Med 2019; (Epub) BAG3-positive pancreatic stellate cells promote migration and invasion of pancreatic ductal adenocarcinoma.
Application : WB, IHC-P
Reactivity : Human
An MX et al. J Cell Biol 2017; (Epub) BAG3 directly stabilizes Hexokinase 2 mRNA and promotes aerobic glycolysis in pancreatic cancer cells.
Application : WB
Reactivity : Mouse
Buhrdel JB et al. Biochem Biophys Res Commun 2015; 461 (2) : 217-23 In-vivo characterization of human myofibrillar myopathy genes in zebrafish.
Application : WB
Reactivity : Human
Submit a Reference
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169