GeneTex
United States (US)

BAG5 antibody, Internal

GTX88490_2036_WB_18122102_399.jpg

Cat No. GTX88490

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88490-PEP).

Specificity/Sensitivity

This antibody is expected to recognise reported isoform a (NP_001015049.1) and isoform, b (NP_001015048.1; NP_004864.1).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence DPQGEEKCKAARKQ, from the internal region of the protein sequence according to NP_001015049.1; NP_001015048.1; NP_004864.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated Athanogene 5,Bag-5,Bag5

Background

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX88490_2036_WB_18122102_399.jpg

GTX88490 WB Image

WB analysis of Jurkat lysate using GTX88490 BAG5 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329