*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , HeLa , Molt-4 , Raji , mouse liver
Rat, Bovine, Chicken, Chimpanzee(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.68 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human BAG5. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Bcl2 Associated Athanogene 5,Bag-5,Bag5
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
GTX102356 WB Image
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BAG5 antibody (GTX102356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX102356 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5(GTX102356) antibody at 1:200 dilution.
GTX102356 WB Image
Sample (30 ug of whole cell lysate)
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX102356 diluted at 1:1000