Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BAG5(GTX102356) antibody at 1:200 dilution.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BAG5 antibody (GTX102356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: Hela
B: Molt-4 (GTX27912)
10% SDS PAGE
GTX102356 diluted at 1:1000