Unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BAI3 antibody [HL4095] (GTX642534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BAI3 antibody [HL4095] (GTX642534) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.