GeneTex
United States (US)

BAIAP2 antibody

GTX115421_40667_20150814_IHC_M_18121410_992.jpg
GTX115421_40667_IFA_18121410_461.jpg
GTX115421_40667_20170511_WB_M_R_18121410_781.jpg
GTX115421_40667_WB_18121410_540.jpg

Cat No. GTX115421

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAIAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bai1 Associated Protein 2,Bap2,Flaf3,Irsp53,Baiap2

Cellular Localization

Cytoplasm , Membrane , Cell projection , filopodium , ruffle

Background

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Database

Research Area

DATA IMAGES
GTX115421_40667_20150814_IHC_M_18121410_992.jpg

GTX115421 IHC-P Image

BAIAP2 antibody detects BAIAP2 protein at cytoplasm in Purkinje cells of mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BAIAP2 antibody (GTX115421) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115421_40667_IFA_18121410_461.jpg

GTX115421 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using BAIAP2(GTX115421) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115421_40667_20170511_WB_M_R_18121410_781.jpg

GTX115421 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAIAP2 antibody (GTX115421) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115421_40667_WB_18121410_540.jpg

GTX115421 WB Image

Sample (30 µg of whole cell lysate)
A: SK-N-SH
7.5% SDS PAGE
GTX115421 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

BAIAP2 antibody Cat No. GTX115421

Rating ( Average 3 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 02-Dec-2016
Score :
Application Tested : IP
Sample Species : Ms
Sample : C2C12 cells
Amount used : 1mg for IP; 20μg for WB
Blocking : 5% skim milk in 0.05% TBST
Primary Antibody : 1:1000, 4°C, 2Hr
Lane Description : BAIAP2 antibody immunoprecipitates BAIAP2 protein in IP experiments. Input. 20 μg C2C12 whole cell extract. IP-antibodies: BAIAP2 antibody(GTX115421), rabbit IgG (control), and nothing (0 ug) (control).
Figure Note : 3ug for IP
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169