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BAIAP2 antibody

Anti-BAIAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115421
Anti-BAIAP2 antibody used in Western Blot (WB). GTX115421
Anti-BAIAP2 antibody used in Western Blot (WB). GTX115421
Anti-BAIAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115421

Cat. No. GTX115421

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BAIAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat brain

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BAIAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAI1 associated protein 2 , BAP2 , FLAF3 , IRSP53

Cellular Localization

Cytoplasm , Membrane , Cell projection , filopodium , ruffle

Background

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Database

Research Area

DATA IMAGES

Anti-BAIAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115421

GTX115421 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using BAIAP2(GTX115421) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-BAIAP2 antibody used in Western Blot (WB). GTX115421

GTX115421 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAIAP2 antibody (GTX115421) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BAIAP2 antibody used in Western Blot (WB). GTX115421

GTX115421 WB Image

Sample (30 μg of whole cell lysate)
A: SK-N-SH
7.5% SDS PAGE
GTX115421 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BAIAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115421

GTX115421 IHC-P Image

BAIAP2 antibody detects BAIAP2 protein at cytoplasm in Purkinje cells of mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
BAIAP2 antibody (GTX115421) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

BAIAP2 antibody Cat. No. GTX115421

Rating
( Average 3 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 3 based on 1 users reviews)
Anti-BAIAP2 antibody used in Immunoprecipitation (IP). GTX115421
Date : Anonymous submitted on 02-Dec-2016
Score :
Application Tested : IP
Sample Species : Ms
Sample : C2C12 cells
Amount used : 1mg for IP; 20μg for WB
Blocking : 5% skim milk in 0.05% TBST
Primary Antibody : 1:1000, 4°C, 2Hr
Lane Description : BAIAP2 antibody immunoprecipitates BAIAP2 protein in IP experiments. Input. 20 μg C2C12 whole cell extract. IP-antibodies: BAIAP2 antibody(GTX115421), rabbit IgG (control), and nothing (0 ug) (control).
Figure Note : 3ug for IP
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169